Tag

Part:BBa_J70627:Design

Designed by: Joseph Lynch   Group: Knight Lab   (2010-08-11)

RFC12 Internal Chitin Binding Domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Both sequences in 5' -> 3' direction.

Forward Primer: 

GTTTCTT C GAATTC GCGGCCGC T ACTAGT ACGACAAATCCTGGTGTATCCGCTTG

Reverse Primer: 

GTTTCTT C CTGCAG CGGCCGC  T GCTAGC TTGAAGCTGCCACAAGGCAGGAAC

Source

NEB plasmid: [http://www.neb.com/nebecomm/products/productN6702.asp pTYB2]

References